FAQ about genetic tests for coronavirus SARS-CoV-2
What are the recommendations of the World Health Organization (WHO) on genetic testing for the coronavirus causing COVID-19?
In order to diagnose infection with the coronavirus SARS-CoV-2, WHO recommends confirmation the viral genetic material presence in the biological material collected from the patient. The only recommended method of determining acute infection is direct detection of the pathogen by means of the virus genetic material amplification, i.e. NAAT (Nucleic Acid Amplification Technology).
What method does Genomtec® SARS-CoV-2 EvaGreen® RT-LAMP CE-IVD Duo Kit test use?
The Genomtec® SARS-CoV-2 EvaGreen® RT-LAMP CE-IVD Duo Kit test is a CE-IVD laboratory kit recognizing specific SRAS-CoV-2 nucleic acid fragment that is amplified using Isothermal Nucleic Acid Amplification Technology (INAAT) with LAMP primers. INAAT is one of the nucleic acid amplification techniques within the NAAT group.
What is the difference between the RT-LAMP and the RT-PCR methods?
- In both of these techniques, there is a reverse transcription reaction and thermocycler is required
- During the RT-PCR reaction, rapid changes in temperature are required during each of the 30-40 cycles in the range from 45°C to 95°C, and the entire analysis process, depending on the material tested and the reagents used, lasts from 1.5 to 3 hours
- In the RT-LAMP technique, the process of amplifying the genetic material takes place at a constant temperature and usually takes about 30 minutes; RT-LAMP method is more sensitive and requires less test material than RT-PCR method
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